Whilst we haven't analyzed PneumaCult™ media to be used with murine epithelial cells, you will find publications that report this application.
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Set the “unstained” tube apart. For that “viability dye” sample, 가슴수술 centrifuge the tube at three hundred x g
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Notes: STEMdiff™ Kidney Organoid Package requires a different plate structure/culture established-up and is not a normal monolayer culture; kidney organoids protrude through the underlying monolayer tradition.
To evaluate the regional specificity of your smaller vs substantial airway, it is possible to execute the following assays:
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Perform cell counts making use of an automated cell counter. Notice: Very low viability is usually optimized by the length of collagenase or ACCUTASE™ incubation. It may also be optimized by minimizing the amount of triturations.
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The next fluorochrome-conjugated antibodies and dyes are proposed to aid Assessment of 지방흡입 gene-edited CD34+ HSPCs: